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KMID : 0380620180500060660
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Korean Journal of Food Science and Technology
2018 Volume.50 No. 6 p.660 ~ p.664
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Antioxidative and antimelanogenic effects of ethyl acetate fractions of Korean domestic honeys from different floral sources
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Jeong Ha-Ram
Baek Young-su
Kim Dae-Ok
Lee Hyung-Jae
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Abstract
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Honey is known to have a variety of health-promoting effects. Ethyl acetate fractions (EAFs) of four Korean domestic honeys from basswood, Korean raisin, chestnut, and acacia as well as a New Zealand manuka honey were analyzed comparatively to evaluate their antioxidative and antimelanogenic effects. The EAFs of basswood, Korean raisin, and chestnut honey had higher antioxidant capacities and tyrosinase inhibition activities than those of manuka honey. Pretreatment of B16F1 melanoma cells with EAFs at 100 µg/mL resulted in relative retention of melanin contents as follows: acacia honey (141.0%)＞basswood honey (134.3%)＞manuka honey (131.5%)＞Korean raisin honey (107.3%)＞chestnut honey (88.0%). Intracellular tyrosinase activities of B16F1 melanoma cells were significantly (p＜0.05) lowered by EAFs of Korean raisin and chestnut honey than by EAF of manuka honey. Consequently, these results suggest that Korean domestic honeys from different floral sources serve as good sources of antioxidative and antimelanogenic agents.
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KEYWORD
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antioxidant capacity, B16F1 melanoma cell, melanin contents, Korean domestic honey, tyrosinase inhibition
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